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Part:BBa_K1758203:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-29)


His-tagged arsenic repressor fused to superfolder GFP under the control of T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 421


Design Notes

We clone arsR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.


Source

This is a composite part which enables a high protein expression and an easy purification of arsR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.

References